GlycoStore

The aim of the MIRAGE project is to improve the quality of glycomics data in the scientific literature by focusing on the development of publication and data reporting guidelines. The MIRAGE-LC module covers all aspects of instrument setup and modality of data handling and manipulation cross-linked with other MIRAGE recommendations.

Example Human IgG Standard

This section provides an example Human IgG glycan dataset reported in compliance with the MIRAGE Guidelines For each glycan the elution time and panel of sequential exoglycosidases (ABS, α2–3,6,8,9 sialidase; BTG, β1-3,4 galactosidase; BKF, α1-2,3,4,6 fucosidase) used to quantitate and confirm structural assignments is provided using a PGC-LC-MS/MS workflow.

No.	GlyTouCan	GlycoStore	RT (min)	Relative % Area	Exoglycosidase	m/z calc	m/z obs	MS2
1	G59471TH	GS734	29.8	0.38	ABS+BTG+BKF	759.8	759.7	Y
2	G96014TN	GS778	32.4	0.45	ABS+BTG	840.8	840.8	N
3	G62880PC	GS736	36.1	5.02	ABS+BTG+BKF	832.8	832.8	Y
4	G52231BF	GS826	37.8	0.27	ABS	1067.4	1067.4	N
5	G39213VZ	GS759	37.8	0.25	ABS+BTG+BKF	658.2	658.2	N
6	G85767HW	GS637	38.1	4.67	ABS+BTG	913.8	913.8	Y
7	G53000RQ	GS943	39.5	1.52	ABS+BTG	994.9	994.9	Y
8	G89993FE	GS632	39.9	0.96	ABS+BTG	739.3	739.3	N
9	G50779LX	GS648	39.9	0.14	ABS	1212.9	1212.9	N
10	G21393DK	GS100026	40.1	0.79	ABS	1059.4	1059.4	Y
11	G66937TJ	GS937	41	0.65	ABS+BTG	739.3	739.3	N
12	G15826PZ	GS825	42.1	1.20	ABS	1140.4	1140.4	Y
13	G36191CD	GS235	42.9	1.03	ABS+BTG	820.3	820.3	Y
14	G09629PQ	GS100025	43.4	0.45	ABS	1285.9	1285.9	Y
15	G69812OF	GS735	43.8	22.28	ABS+BTG+BKF	731.3	731.2	Y
16	G18609BT	GS941	44.8	0.53	ABS	965.8	965.8	Y
17	G05546WG	GS630	45.6	19.88	ABS+BTG	812.3	812.3	Y
18	G84467IZ	GS100022	46	0.27	ABS	1111.4	1111.4	N
19	G09782JM	GS630	46.3	12.64	ABS+BTG	812.3	812.3	Y
20	G33284MM	GS816	47.7	2.06	ABS	957.8	957.8	Y
21	G28826SH	GS731	47.9	15.81	ABS+BTG	893.3	893.3	Y
22	G62689JX	GS683	49.1	7.79	ABS	1038.9	1038.9	Y
23	G83594ZB	GS100017	50	0.98	ABS	1184.4	1184.4	Y