Report - Ludger
101 glycan structures
| Glycan | Mono Mass | UPLC (2-AB) | UPLC (Proc) | HPLC | RPUPLC | CE |
|---|---|---|---|---|---|---|
M3 |
910.33 | 4.31 ± 0.06 | 4.17 | 4.41 ± 0.13 | 4.83 ± 0.02 | |
F(6)A2[3]G(4)1 |
1624.6 | 6.83 ± 0.031 | 6.6 ± 0.099 | 6.79 ± 0.067 | 9.06 ± 0.055 | |
F(6)A2[3]BG(4)1 |
1827.68 | 7.08 ± 0.016 | 6.92 ± 0.071 | 9.56 ± 0.008 | ||
A2G(4)2 |
1640.59 | 7.18 ± 0.038 | 6.93 ± 0.067 | 7.15 ± 0.033 | 9.11 ± 0.036 | |
A2[6]G(4)1 |
1478.54 | 6.28 ± 0.05 | 6.03 ± 0.028 | 6.27 ± 0.058 | 7.74 ± 0.017 | |
A3G(4)3 |
2005.72 | 8.4 ± 0.106 | 8.21 | 8.36 ± 0.035 | 11.58 ± 0.022 | |
NeuNAcα2-3Galβ1-3GalNAc |
674.24 | 2.94 ± 0.047 | ||||
A2[6]BG(4)1 |
1681.62 | 6.55 ± 0.02 | 6.36 ± 0.068 | 8.24 ± 0.008 | ||
Galβ1-4GlcNAcβ1-6[Galβ1-3]GalNAc |
748.27 | 3.58 ± 0.012 | ||||
F(6)A2[6]G(4)1 |
1624.6 | 6.72 ± 0.033 | 6.48 ± 0.093 | 6.65 ± 0.048 | 8.72 ± 0.059 | |
Fucα1-2Gal |
326.12 | 1.57 ± 0.006 | ||||
F(6)A1 |
1259.47 | 5.4 ± 0.025 | 5.1 | 5.44 ± 0.138 | ||
F(6)A2[6]BG(4)1 |
1827.68 | 6.95 ± 0.018 | 6.83 ± 0.074 | 9.1 ± 0.049 | ||
F(6)M3 |
1056.39 | 4.57 ± 0.014 | 4.62 | 4.88 ± 0.049 | 5.58 ± 0.04 | |
A2 |
1316.49 | 5.44 ± 0.038 | 5.25 ± 0.064 | 5.43 ± 0.051 | 6.67 ± 0.08 | |
F(6)A3 |
1665.62 | 6.28 ± 0.114 | 6.05 | 6.36 ± 0.167 | ||
Galβ1-3GlcNAc |
383.14 | 1.8 | ||||
A2G(3)2 |
1640.59 | 7.0 | ||||
Fucα1-2[GalNAcα2-3]Gal |
529.2 | 2.5 | ||||
Fucα1-2Galβ1-3GalNAc |
529.2 | 2.3 | ||||
A4G(4)4 |
2370.86 | 9.78 ± 0.072 | 9.67 ± 0 | 13.55 ± 0.023 | ||
A3G(3)1 |
1681.62 | 6.6 | ||||
GalNAcα1-3Gal |
383.14 | 2.17 ± 0.173 | ||||
A2G(3)1 |
1478.54 | 6.2 | ||||
NeuNAcα2-6[NeuNAcα2-3Galβ1-3]GalNAc |
965.33 | 4.4 | ||||
NeuNAcα2-6[Galβ1-3]GalNAc |
674.24 | 3.26 | ||||
F(6)A1[6]G(4)1Ga(3)1 |
1583.57 | 6.8 | ||||
F(6)A2BG(4)1 |
1827.68 | 6.84 ± 0.05 | 6.74 ± 0.099 | 6.9 ± 0.049 | ||
F(6)A3G(4)1 |
1827.68 | 6.66 | ||||
A2G(4)2S(6,6)2 |
2222.78 | 9.47 ± 0.299 | 9.04 ± 0.148 | 8.82 ± 0.404 | 4.55 ± 0.03 | |
A2BG(4)2S(6,6)2 |
2425.86 | 9.73 ± 0.228 | 9.13 | 9.04 ± 0 | 4.69 ± 0.035 | |
F(6)A2G(4)2S(6,6)2 |
2368.84 | 10.0 ± 0.147 | 9.39 ± 0.184 | 9.0 ± 0.243 | 4.85 ± 0.036 | |
A3G3 |
2005.72 | 8.29 | 8.35 ± 0.112 | |||
M4A1 |
1275.46 | 5.89 ± 0.022 | 5.54 | 6.0 ± 0 | ||
A2G(4)2Ga(3)2 |
1964.7 | 9.06 | ||||
F(6)A3G(4)2Ga(3)1 |
2151.78 | 8.98 | ||||
A3G(4)3Ga(3)1 |
2167.78 | 9.44 | ||||
A3G(4)2Ga(3)2 |
2167.78 | 9.42 | ||||
F(6)A2G(4)2Ga(3)1 |
1948.7 | 8.6 | ||||
F(6)A3G(4)3Ga(3)2 |
2475.89 | 10.96 | ||||
F(6)A3G(4)2Ga(3)2 |
2313.84 | 9.8 | 9.39 | |||
A2G(4)1S(6)1 |
1769.63 | 7.95 | 7.2 | |||
F(6)A3G(4)3 |
2151.78 | 8.63 ± 0.127 | 8.57 | 8.83 ± 0.104 | 12.5 ± 0.016 | |
F(6)A2G(4)2Ga(3)2 |
2110.76 | 9.51 | 9.12 | |||
A3G(4)3Ga(3)2 |
2329.83 | 10.56 | ||||
F(6)A3G(4)3Ga(3)1 |
2313.84 | 10.17 | 9.39 | |||
A2BG(4)1 |
1681.62 | 6.37 | 6.51 ± 0.04 | |||
M4 |
1072.38 | 5.24 ± 0.075 | 5.0 | 5.28 ± 0.111 | ||
A2[3]G(4)1 |
1478.54 | 6.39 ± 0.028 | 6.14 | 6.45 ± 0.083 | 8.04 ± 0.018 | |
F(6)A2G(4)1S(6)1 |
1915.69 | 8.08 | 7.54 | 7.67 | ||
A2[3]BG(4)1 |
1681.62 | 6.7 ± 0.02 | 6.52 ± 0.071 | 8.54 ± 0.01 | ||
A1[6]G(4)1Ga1 |
1437.51 | 6.74 | ||||
F(3)A3 |
1665.62 | 6.38 | ||||
A2[6]G(4)1Ga(3)1 |
1640.59 | 7.18 | ||||
A1[3]G(4)1Ga1 |
1437.51 | 6.86 | ||||
A3G(4)2Ga(3)1 |
2005.72 | 8.4 | ||||
A2G(4)2Ga(3)1 |
1802.64 | 8.18 | ||||
A3G(4)1Ga(3)1 |
1843.67 | 7.65 | ||||
A2[3]G(4)1Ga(3)1 |
1640.59 | 7.37 | ||||
F(6)A3G(4)1Ga(3)1 |
1989.73 | 8.1 | 7.72 | |||
A2G(4)2S(6)1 |
1931.69 | 8.29 ± 0.13 | 8.0 ± 0.148 | 7.86 ± 0.26 | 6.11 ± 0.047 | |
F(6)A2[3]G(4)1Ga(3)1 |
1786.65 | 7.83 | 7.46 | |||
F(6)A2BG(4)2 |
1989.73 | 7.75 ± 0.03 | 7.57 ± 0.191 | 7.67 ± 0.043 | 10.46 ± 0.056 | |
A3 |
1519.57 | 5.88 ± 0.041 | 5.87 ± 0.039 | 7.81 ± 0.025 | ||
NeuNAcα2-3Gal |
471.16 | 2.25 ± 0.041 | ||||
M5A1 |
1437.51 | 6.66 ± 0.022 | 6.46 | 6.6 ± 0.018 | ||
F(6)A2BG(4)2S(6)1 |
2280.82 | 8.99 ± 0.092 | 8.62 ± 0.141 | 8.56 ± 0.105 | 6.77 ± 0.058 | |
F(6)A3G(4)2 |
1989.73 | 7.65 ± 0.057 | 7.85 | |||
M2 |
748.27 | 3.21 | 3.48 ± 0.028 | |||
F(6)A1[3]G(4)1Ga(3)1 |
1583.57 | 7.0 | ||||
A3G(4,4,3)3 |
2005.72 | 3.0 | 8.08 | 8.3 | ||
A2G(4)1Ga(3)1 |
1640.59 | 6.91 | ||||
F(6)A2[3]G(4)1Sg1 |
1931.69 | 8.1 | ||||
F(6)A1G(4)1 |
1421.52 | 6.4 ± 0.012 | 6.05 | 6.19 | ||
F(6)A2[6]G(4)1Sg1 |
1931.69 | 7.97 | ||||
F(6)A3G(4)3Ga(3)3 |
2637.94 | 11.17 | ||||
A2G(4)1 |
1478.54 | 6.42 ± 0.216 | ||||
A2G(4)2Sg1 |
1947.68 | 10.11 | ||||
F(6)A2G(4)2[3]Ga1 |
1948.7 | 8.45 | ||||
F(6)A2G(4)2[6]Ga1 |
1948.7 | 8.22 | ||||
A1 |
1113.41 | 4.93 ± 0.538 | 4.76 | 4.96 ± 0.048 | ||
F(6)A2G(4)2Ga(3)1Sg1 |
2255.79 | 9.56 ± 0.24 | ||||
F(6)A2G(4)2Sg1 |
2093.74 | 9.7 ± 1.138 | ||||
A1B |
1316.49 | 5.33 ± 0.086 | 5.33 ± 0.036 | |||
M5 |
1234.43 | 6.21 ± 0.047 | 5.95 ± 0.081 | 6.17 ± 0.033 | 6.75 ± 0.014 | |
F(6)A2G(4)2S(6)1 |
2077.75 | 8.71 ± 0.155 | 8.39 ± 0.138 | 8.29 ± 0.233 | 6.61 ± 0.061 | |
A3G(4)2 |
1843.67 | 7.37 ± 0.231 | ||||
A1[6] |
1113.41 | 4.72 | 4.92 ± 0.021 | 5.75 ± 0.005 | ||
A2BG(4)2 |
1843.67 | 7.36 ± 0.024 | 7.3 ± 0.056 | 9.56 ± 0.05 | ||
M6 |
1396.49 | 7.03 ± 0.053 | 6.85 ± 0.059 | 7.0 ± 0.068 | 7.6 ± 0.005 | |
M9 |
1882.64 | 9.53 ± 0.045 | 9.35 | 9.52 ± 0.069 | 10.13 ± 0.013 | |
NeuNAcα2-3Galβ1-4GlcNAcβ1-6[NeuNAcα2-3Galβ1-3]GalNAc |
1330.47 | 5.4 ± 0.015 | ||||
F(6)A2BG(4)2S(6,6)2 |
2571.92 | 10.13 ± 0.148 | 9.54 ± 0.148 | 9.05 ± 0.215 | 4.91 ± 0.033 | |
F(6)A2G(4)2 |
1786.65 | 7.57 ± 0.05 | 7.35 ± 0.135 | 7.56 ± 0.033 | 10.1 ± 0.051 | |
A2B |
1519.57 | 5.79 ± 0.019 | 5.74 ± 0.056 | 7.18 ± 0.046 | ||
F(6)A2 |
1462.54 | 5.91 ± 0.048 | 5.66 ± 0.076 | 5.88 ± 0.038 | 7.63 ± 0.064 | |
F(6)A2B |
1665.62 | 6.24 ± 0.025 | 6.06 ± 0.071 | 6.19 ± 0.034 | 8.13 ± 0.059 | |
M8 |
1720.59 | 8.9 | 8.59 ± 0.092 | 8.85 ± 0.169 | 9.51 ± 0.005 | |
M7 |
1558.54 | 7.86 ± 0.013 | 7.79 | 7.94 ± 0.05 | 8.64 ± 0.005 | |
NeuNAcα2-6GalNAc |
471.16 | 2.42 ± 0.028 | ||||
M3B |
1113.41 | 4.89 ± 0.037 | 4.96 ± 0.021 |
Filter by
O-glycan
References
Data Source
Human IgG Glycoprotein Standard, Commercial Standards, GCP-IgG, GCP-Fet, tsetse fly saliva
Methods
2-AB labelled samples were analysed by HILIC-UPLC using an ACQUITY UPLC® BEH-Glycan 1.7 μm, 2.1 x 150 mm column on an ACQUITY UPLC H-Class instrument
Buffer A was 50 mM ammonium formate made from Ludger stock buffer, Buffer B was acetonitrile. Samples were injected in 28% aqueous/72% acetonitrile (28 μL sample + 72 μL acetonitrile); injection volume 25μL. Gradient conditions were: 0-27min, 28-38%A; 27-30min, 38-100%A; 30-31min,100% A; 31-32min, 100-28%A; 32-35min, 28%A; at a flow rate of 0.4 mL/min.
UniCarbKB GlyTouCan